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Sulfo-Cyanine3 NHS Ester【CAS 1424150-38-8】
NMR COA MSDS - Names：
Sulfo-Cyanine3 NHS Ester
- CAS號：
1424150-38-8
MDL Number： - MF(分子式)： C34H38N3KO10S2 MW(分子量)： 735.8
- EINECS:Not available Reaxys Number:No data available
- Pubchem ID:154731801 Brand:BIOFOUNT
Sulfo-Cyanine3 NHS Ester(1424150-38-8)是一種有效的可以使用純水溶液標記的蛋白質和肽，無需有機助溶劑, Sulfo-Cyanine3 NHS Ester 適合標記蛋白等對有機溶劑敏感的生物分子，也適用于溶解度低的蛋白質和易于變性的蛋白質。因為磺化Cy3極好水溶性特點，且染料分子本身帶電荷，標記到蛋白表面后不會引發疏水性蛋白聚集，可以提高熒光標記產物的穩定性。ex/em (PBS): 550/566 nm
| 貨品編碼 | 規格 | 純度 | 價格 (￥) | 現價(￥) | 特價(￥) | 庫存描述 | 數量 | 總計 (￥) |
|---|---|---|---|---|---|---|---|---|
| HCC345498-5mg | 5mg | 97% | ￥ 1530.00 | ￥ 1530.00 | 2-3days | ￥ 0.00 | ||
| HCC345498-1mg | 1mg | 97% | ￥ 720.00 | ￥ 720.00 | 2-3days | ￥ 0.00 |
| 中文別名 | Sulfo-Cyanine3 NHS Ester;磺酸基Cy3活性酯:(1424150-38-8);磺化Cy3-NHS酯；Sulfo Cyanine3 NHS ester;磺化Cy3-NHS 活化酯;磺化Cy3-N-羥基琥珀酰亞胺酯 |
| 英文別名 | 1424150-38-8;Sulfo-Cyanine3 NHS Ester;Sulfo Cyanine3 NHS Ester；1424150-38-8 (sodium salt); 1424433-17-9, 1518643-34-9 (inner salt) |
| CAS號 | 1424150-38-8 |
| Inchi | InChI=1S/C34H39N3O10S2.K/c1-33(2)24-20-22(48(41,42)43)13-15-26(24)35(5)28(33)10-9-11-29-34(3,4)25-21-23(49(44,45)46)14-16-27(25)36(29)19-8-6-7-12-32(40)47-37-30(38)17-18-31(37)39;/h9-11,13-16,20-21H,6-8,12,17-19H2,1-5H3,(H-,41,42,43,44,45,46);/q;+1/p-1 |
| InchiKey | ZNWGQSGULWNMHO-UHFFFAOYSA-M |
| 分子式 Formula | C34H38N3KO10S2 |
| 分子量 Molecular Weight | 735.8 |
| 溶解度Solubility | Soluble to water，溶于水 (0.62 M = 47 g/L)，溶于極性有機溶劑 (DMF, DMSO) |
| 性狀 | solid,紅色固體；dark red crystals |
| 儲藏條件 Storage conditions | storage at -4℃ (1-2weeks), longer storage period at -20℃ (1-2years) |
Sulfo-Cyanine3 NHS Ester說明：
A variety of cyanine dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy3® dyes are one type of the most common red fluorophores. These versatile fluorophores can tolerate a pH range of 3-10 for use in a variety of applications at biologically relevant pHs. The dyes are also DMSO tolerant and photostable to enable transfer from storage to assay without loss of performance. The aqueous solubility eliminates the need for organic solvents in the assay buffers. Sulfo-Cyanine 3 is an alternative dye primarily used for labeling peptides and oligos in replacing Cy3 that was originally developed by GE Healthcare (Cy3® is the trademark of GE Healthcare). NHS ester dyes are recommended for labeling amine groups and maleimide dyes are recommended for labeling thiol groups. This Cy3® NHS ester readily reacts with amino groups.
Sulfo-Cyanine3 NHS Ester 可替代：Cy3®、Alexa Fluor 546 和 DyLight 549
| Molecular weight 735.19 |
Correction Factor (260 nm) 0.07 |
Correction Factor (280 nm) 0.073 |
Extinction coefficient (cm -1 M -1) 1500001 |
Excitation (nm) 555 |
Emission (nm) 569 |
1.實驗前需戴好防護眼鏡，穿戴防護服和口罩，佩戴手套，避免與皮膚接觸。
2.實驗過程中如遇到有毒或者刺激性物質及有害物質產生，必要時實驗操作需要手套箱內完成以免對實驗人員造成傷害
3.實驗后產生的廢棄物需分類存儲，并交于專業生物廢氣物處理公司處理，以免造成環境污染Experimental considerations:
1. Wear protective glasses, protective clothing and masks, gloves, and avoid contact with the skin during the experiment.
2. The waste generated after the experiment needs to be stored separately, and handed over to a professional biological waste gas treatment company to avoid environmental pollution.
| 產品說明 | Sulfo-Cyanine3 NHS Ester(1424150-38-8)是一種有效的可以使用純水溶液標記的蛋白質和肽，無需有機助溶劑, Sulfo-Cyanine3 NHS Ester 適合標記蛋白等對有機溶劑敏感的生物分子，也適用于溶解度低的蛋白質和易于變性的蛋白質。 |
| Introduction | Water soluble, amino-reactive sulfo-Cyanine3 NHS ester. Efficiently labels proteins and peptides in purely aqueous solution, without need for organic co-solvent. Ideal for proteins with low solubility |
| Application1 | |
| Application2 | |
| Application3 |
| 警示圖 | |
| 危險性 | warning |
| 危險性警示 | Not available |
| 安全聲明 | H303+H313+H333 |
| 安全防護 | P264+P280+P305+P351+P338+P337+P313 |
| 備注 | 實驗過程中防止吸入、食入，做好安全防護 |
| 1. Xu, D.; Wang, Y. smFRET study of rRNA dimerization at the peptidyl transfer center. Biophysical Chemistry, 2021, 277, 106657. doi: 10.1016/j.bpc.2021.106657 |
| 2. Taghian, T.; Metelev, V.G.; Zhang, S.; Bogdanov, A.A. Imaging NF-κB activity in a murine model of early stage diabetes. FASEB Journal, 2020, 34(1), 1198–1210. doi: 10.1096/fj.201801147r |
| 3. Ni, C.-W.; Wei, Y.-J.; Shen, Y.-I.; Lee, I.-R. Long-Range Hairpin Slippage Reconfiguration Dynamics in Trinucleotide Repeat Sequences. The Journal of Physical Chemistry Letters, 2019, 10, 3985–3990 |
| 4. Cao, P.; Wei, X.; Awal, R.P.; Müller, R.; Wall, D. A Highly Polymorphic Receptor Governs Many Distinct Self-Recognition Types within the Myxococcales Order. mBio, 2019, 10, e02751-18. |
| 5. Sallada, N.D.; Dunn, K.J.; Berger, B.W. A structural and functional role for disulfide bonds in a class II hydrophobin. Biochemistry, 2018, 57(5), 645–653. doi: 10.1021/acs.biochem.7b01166 |
6. Park, B.G.; Kim, Y.J.; Min, J.H.; Cheong, T.-C.; Nam, S.H.; Cho, N.-H.; Kim, Y.K.; Lee, K.B. Assessment of Cellular Uptake Efficiency According to Multiple Inhibitors of Fe3O4-Au Core-Shell Nanoparticles: Possibility to Control Specific Endocytosis in Colorectal Cancer Cells. Nanoscale Research Letters, 2020, 15, 165. doi: 10.1186/s11671-020-03395-w
7. Wang, C.; Fernández de Ávila, B.E.; Mundaca-Uribe, R.; Lopez-Ramirez, M.A.; Ramírez-Herrera, D.E.; Shukla, S.; Steinmetz, N.F.; Wang, J. Active Delivery of VLPs Promotes Anti-Tumor Activity in a Mouse Ovarian Tumor Model. Small, 2020, 16(20), e1907150. doi: 10.1002/smll.201907150
8. Saez Talens, V.; Arias-Alpizar, G.; Makurat, D.M.M.; Davis, J.; Bussmann, J.; Kros, A.; Kieltyka, R.E. Stab2-Mediated Clearance of Supramolecular Polymer Nanoparticles in Zebrafish Embryos. Biomacromolecules, 2020, 21(3), 1060–1068. doi: 10.1021/acs.biomac.9b01318
9. Choi, J.; Marks, J.; Zhang, J.; Chen, D.-H.; Wang, J.; Vázquez-Laslop, N.; Mankin, A.S:; Puglisi, J.D. Dynamics of the context-specific translation arrest by chloramphenicol and linezolid. Nature Chemical Biology, 2020, 16, 310–317. doi: 10.1038/s41589-019-0423-2
10. Song, P.; Shen, J.; Ye, D.; Dong, B.; Wang, F.; Pei, H.; Wang, J.; Shi, J.; Wang, L.; Xue, W.; Huang, Y.; Huang, G.; Zuo, X.; Fan, C. Programming bulk enzyme heterojunctions for biosensor development with tetrahedral DNA framework. Nature Communications, 2020, 11, 838. doi: 10.1038/s41467-020-14664-8
7. Wang, C.; Fernández de Ávila, B.E.; Mundaca-Uribe, R.; Lopez-Ramirez, M.A.; Ramírez-Herrera, D.E.; Shukla, S.; Steinmetz, N.F.; Wang, J. Active Delivery of VLPs Promotes Anti-Tumor Activity in a Mouse Ovarian Tumor Model. Small, 2020, 16(20), e1907150. doi: 10.1002/smll.201907150
8. Saez Talens, V.; Arias-Alpizar, G.; Makurat, D.M.M.; Davis, J.; Bussmann, J.; Kros, A.; Kieltyka, R.E. Stab2-Mediated Clearance of Supramolecular Polymer Nanoparticles in Zebrafish Embryos. Biomacromolecules, 2020, 21(3), 1060–1068. doi: 10.1021/acs.biomac.9b01318
9. Choi, J.; Marks, J.; Zhang, J.; Chen, D.-H.; Wang, J.; Vázquez-Laslop, N.; Mankin, A.S:; Puglisi, J.D. Dynamics of the context-specific translation arrest by chloramphenicol and linezolid. Nature Chemical Biology, 2020, 16, 310–317. doi: 10.1038/s41589-019-0423-2
10. Song, P.; Shen, J.; Ye, D.; Dong, B.; Wang, F.; Pei, H.; Wang, J.; Shi, J.; Wang, L.; Xue, W.; Huang, Y.; Huang, G.; Zuo, X.; Fan, C. Programming bulk enzyme heterojunctions for biosensor development with tetrahedral DNA framework. Nature Communications, 2020, 11, 838. doi: 10.1038/s41467-020-14664-8
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