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Fluo-8, AM鈣離子熒光探針【CAS 1345980-40-6】
NMR COA MSDS
Names：
CalciFluor Fluo-8, AM
CAS號：
1345980-40-6
MDL Number：
MF(分子式)： C50H50N2O23 MW(分子量)： 1046.93
EINECS: Reaxys Number:
Pubchem ID:54575324 Brand:BIOFOUNT

Fluo-8, AM鈣離子熒光探針;(CalciFluor™ Fluo-8, AM) 是一種綠色熒光鈣結(jié)合染料，是一種優(yōu)于傳統(tǒng) Fluo-4, AM 的 Ca2+ 熒光探針。熒光 : λex 494 nm, λem 517 nm in DMSO

貨品編碼	規(guī)格	純度	價格 (￥)	現(xiàn)價(￥)	特價(￥)	庫存描述	數(shù)量	總計 (￥)
HCM0029B-1mg	1mg	98%ab	￥ 0.00	￥ 0.00	4800	2-3weeks	- +	￥ 0.00

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中文別名	鈣離子熒光探針Fluo-8, AM;鈣離子熒光探針；鈣熒光探針Fluo-8 AM；鈣離子探針；鈣熒光探針;鈣熒光探針 Fluo-8 AM
英文別名	CalciFluor Fluo-8, AM；Fluo-8, AM；Fluo-8 AM；Bis(acetoxymethyl) 2,2'-((4-(6-(acetoxymethoxy)-3-oxo-3H-xanthen-9-yl)-2-(2-(bis(2-acetoxymethoxy)-2-oxoethyl)amino)phenoxy)ethoxy)phenyl)azanediyl)diacetate
CAS號	1345980-40-6
Inchi	InChI=1S/C50H50N2O23/c1-30(53)65-25-70-37-12-14-39-44(20-37)75-43-19-36(58)11-13-38(43)50(39)35-10-15-41(52(23-48(61)73-28-68-33(4)56)24-49(62)74-29-69-34(5)57)45(18-35)64-17-16-63-42-9-7-6-8-40(42)51(21-46(59)71-26-66-31(2)54)22-47(60)72-27-67-32(3)55/h6-15,18-20H,16-17,21-29H2,1-5H3
InchiKey	BKYHTWFRZPRHNM-UHFFFAOYSA-N
分子式 Formula	C50H50N2O23
分子量 Molecular Weight	1046.93
溶解度Solubility	Soluble in DMSO, and water (low).
性狀	Solid power
儲藏條件 Storage conditions	Desiccate at -20° C

Fluo-8, AM 鈣離子熒光探針CalciFluor™ Fluo-8, AM 的應(yīng)用
CalciFluor™ Fluo-8, AM 已開發(fā)用于改善細(xì)胞負(fù)載和鈣響應(yīng)，同時保持便利的 Fluo-3 和 Fluo-4 光譜波長，最大激發(fā) @ ~490 nm 和最大發(fā)射 @ ~520 nm。與 Fluo 4 AM 類似，陰離子羧基被乙酰氧基甲酯保護，允許膜滲透，隨后被細(xì)胞內(nèi)酯酶去除 - 允許 Ca2+ 檢測。

CalciFluor™ Fluo-8, AM has been developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength of maximum excitation @ ~490 nm and maximum emission @ ~520 nm. Similarly to Fluo 4 AM, the anionic carboxy groups are protected with acetoxy methyl esters allowing membrane permeability, which are subsequently removed by intracellular esterases - allowing Ca2+ detection. However CalciFluor™ Fluo-8, AM is efficiently loaded into cells at room temperature (whereas Fluo-3 and Fluo-4 require much more harsh loading conditions), and CalciFluor™ Fluo-8, AM is 2 times brighter than Fluo-4 and 4 times brighter than Fluo-3. These features, as well as a lower price, make CalciFluor™ Fluo-8, AM a superior in vivo calcium indicator.

FLUO-8 (AM) 是 Fluo-8 的一種可滲透細(xì)胞的乙酰氧基甲基 (AM) 酯衍生物。亮度大約是 Fluo-4 的兩倍。吸光度 452 nm (26,000)；發(fā)射 515 nm（AM 水解后；溶于 10 mM EGTA、100 mM KCl、10 mM MOPS，pH 7.2）

Fluo-8 AM, green fluorescent calcium binding dye -- 熒光細(xì)胞成像
U2OS 細(xì)胞在 96 孔黑壁/透明底部 costar 板中以每 100 µL/孔 40,000 個細(xì)胞過夜播種。去除生長培養(yǎng)基，將細(xì)胞與 100 µl 4 µM Fluo-4 AM 或 Fluo-8® AM 在 HHBS 中在 37 °C、5% CO2 培養(yǎng)箱中孵育 1 小時。細(xì)胞用 200 µl HHBS 洗滌兩次，然后使用 FITC 通道在熒光顯微鏡下成像。

產(chǎn)品說明	Fluo-8, AM鈣離子熒光探針;(CalciFluor Fluo-8, AM) 是一種綠色熒光鈣結(jié)合染料，是一種優(yōu)于傳統(tǒng) Fluo-4, AM 的 Ca2+ 熒光探針。熒光 : λex 494 nm, λem 517 nm in DMSO
Introduction	CalciFluor Fluo-8, AM is a green fluorescent calcium binding dye, a superior Ca2+ fluorescent probe than traditional Fluo-4, AM.
Application1
Application2
Application3

Calcium measurements are critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence microplate readers. Fluo-3 AM and Fluo-4 AM are most commonly used among the visible light-excitable calcium indicators for live-cell calcium imaging. However, Fluo-3 AM and Fluo-4 AM are only moderately fluorescent in live cells upon esterase hydrolysis and require harsh cell loading conditions to maximize their cellular calcium responses. Fluo-8® dyes are developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelengths of Ex/Em = ∼490/∼520 nm. Fluo-8® AM can be loaded into cells at room temperature, while Fluo-3 AM and Fluo-4 AM require 37°C for cell loading. In addition, Fluo-8® AM is two times brighter than Fluo-4 AM and four times brighter than Fluo-3 AM. AAT Bioquest offers a set of our outstanding Fluo-8® reagents with different calcium-binding affinities (Fluo-8® Kd = 389 nM; Fluo-8H™ Kd = 232 nM; Fluo-8L™ Kd = 1.86 µM; Fluo-8FF™ Kd = 10 µM). We also offer versatile packing sizes to meet your special needs (e.g., 1 mg, 10x50 µg, 20x50 µg, and HTS packages) with no additional packaging charge.

警示圖
危險性
危險性警示	No data available
安全聲明	H319 (100%): Causes serious eye irritation [Warning Serious eye damage/eye irritation]
安全防護	P264, P280, P305+P351+P338, and P337+P313
備注

1. Liu L et al. TRPM7 promotes the epithelial-mesenchymal transition in ovarian cancer through the calcium-related PI3K / AKT oncogenic signaling. J Exp Clin Cancer Res 38:106 (2019).

2. Domínguez A et al. Effect of perfluorodecanoic acid on pig oocyte viability, intracellular calcium levels and gap junction intercellular communication during oocyte maturation in vitro. Toxicol In

3. Bender BJ et al. Structural Model of Ghrelin Bound to its G Protein-Coupled Receptor. Structure 27:537-544.e4 (2019).

4. Datta G et al. HIV-1 gp120 Promotes Lysosomal Exocytosis in Human Schwann Cells. Front Cell Neurosci 13:329 (2019).

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